Electrocutaneous stimulation to close the loop in myoelectric prosthesis control

Current commercially available prosthetic systems still lack sensory feedback and amputees are forced to maintain eye-contact with the prosthesis when interacting with their environment. Electrocutaneous stimulation is a promising approach to convey sensory feedback via the skin. However, when discussed in the context of prosthetic applications, it is often refused due to its supposed incompatibility with myocontrol. This dissertation now addresses electrocutaneous stimulation as means to provide sensory feedback to prosthesis users, and its implications on myoelectric control, possible use for improved or accelerated mastering of prosthesis control through closing of the control loop, as well as its potential in aiding in the embodiment of prosthetic components. First, a comparison of different paradigms for encoding sensory feedback variables in electrocutaneous stimulation patterns was done. For this, subject ability to employ spatially and intensity-coded electrocutaneous feedback in a simulated closed-loop control task was evaluated. The task was to stabilise an invisible virtual inverted pendulum under ideal feedforward control conditions (joystick). Pendulum inclination was either presented spatially (12 stimulation sites), encoded by stimulation strength (≧ 2 stimulation sites), or a combination of the two. The tests indicated that spatial encoding was perceived as more intuitive, but intensity encoding yielded better performance and lower energy expenditure. The second study investigated the detrimental influence of stimulation artefacts on myoelectric control of prostheses for a wide range of stimulation parameters and two prosthesis control approaches (pattern recognition of eight motion primitives, direct proportional control). Artefact blanking is introduced and discussed as a practical approach to handle stimulation artefacts and restore control performance back to the baseline. This was shown with virtual and applied artefact blanking (pattern recognition on six electromyographic channels), as well as in a practical task-related test with a real prosthesis (proportional control). The information transfer of sensory feedback necessary to master a routine grasping task using electromyographic control of a prosthesis was investigated in another study. Subjects controlled a real prosthesis to repeatedly grasp a dummy object, which implemented two different objects with previously unknown slip and fragility properties. Three feedback conditions (basic feedback on grasp success, visual grasp force feedback, tactile grasp force feedback) were compared with regard to their influence on subjects’ task performance and variability in exerted grasp force. It was found that online force feedback via a visual or tactile channel did not add significant advantages, and that basic feedback was sufficient and was employed by subjects to improve both performance and force variability with time. Importantly, there was no adverse effect of the additional feedback, either. This has important implications for other non-functional applications of sensory feedback, such as facilitation of embodiment of prosthetic devices. The final study investigated the impact of electrocutaneous stimulation on embodiment of an artificial limb. For this purpose, a sensor finger was employed in a rubber-hand-illusion-like experiment. Two independent groups (test, control), were compared with regard to two objective measures of embodiment: proprioceptive drift, and change in skin temperature. Though proprioceptive drift measures did not reveal differences between conditions, they indicated trends generally associated to a successful illusion. Additionally, significant changes in skin temperature between test and control group indicated that embodiment of the artificial digit could be induced by providing sensory substitution feedback on the forearm. In conclusion, it has been shown that humans can employ electrocutaneous stimulation feedback in challenging closed-loop control tasks. It was found that transition from simple intuitive encodings (spatial) to those providing better resolution (intensity) further improves feedback exploitation. Blanking and segmentation approaches facilitate simultaneous application of electrocutaneous stimulation and electromyographic control of prostheses, using both pattern recognition and classic proportional approaches. While it was found that force feedback may not aid in the mastering of routine grasping, the presence of the feedback was also found to not impede the user performance. This is an important implication for the application of feedback for non-functional purposes, such as facilitation of embodiment. Regarding this, it was shown that providing sensory feedback via electrocutaneous stimulation did indeed promote embodiment of an artificial finger, even if the feedback was applied to the forearm. Based on the results of this work, the next step should be integration of sensory feedback into commercial devices, so that all amputees can benefit from its advantages. Electrocutaneous stimulation has been shown to be an ideal means for realising this. Hitherto existing concerns about the compatibility of electrocutaneous stimulation and myocontrol could be resolved by presenting appropriate methods to deal with stimulation artefacts

Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis

Feline idiopathic cystitis (FIC) is the only spontaneous animal model for human interstitial cystitis (IC), as both possess a distinctive chronical and relapsing character. Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin levels in FIC cases. The purpose of this study was to gain further insight into the pathogenesis by assessing interacting partners of fibronectin in urine of FIC affected cats. Several candidate proteins were identified via immunoprecipitation and mass spectrometry. Considerable changes in FIC conditions compared to physiological expression of co-purified proteins were detected by Western blot and immunohistochemistry. Compared to controls, complement C4a and thioredoxin were present in higher levels in urine of FIC patients whereas loss of signal intensity was detected in FIC affected tissue. Galectin-7 was exclusively detected in urine of FIC cats, pointing to an important role of this molecule in FIC pathogenesis. Moderate physiological signal intensity of galectin-7 in transitional epithelium shifted to distinct expression in transitional epithelium under pathophysiological conditions. I-FABP expression was reduced in urine and urinary bladder tissue of FIC cats. Additionally, transduction molecules of thioredoxin, NF-κB p65 and p38 MAPK, were examined. In FIC affected tissue, colocalization of thioredoxin and NF-κB p65 could be demonstrated compared to absent coexpression of thioredoxin and p38 MAPK. These considerable changes in expression level and pattern point to an important role for co-purified proteins of fibronectin and thioredoxin-regulated signal transduction pathways in FIC pathogenesis. These results could provide a promising starting point for novel therapeutic approaches in the future

Complementary limb motion estimation for the control of active knee prostheses

To restore walking after transfemoral amputation, various actuated exoprostheses have been developed, which control the knee torque actively or via variable damping. In both cases, an important issue is to find the appropriate control that enables user-dominated gait. Recently, we suggested a generic method to deduce intended motion of impaired or amputated limbs from residual human body motion. Based on interjoint coordination in physiological gait, statistical regression is used to estimate missing motion. In a pilot study, this complementary limb motion estimation (CLME) strategy is applied to control an active knee exoprosthesis. A motor-driven prosthetic knee with one degree of freedom has been realized, and one above-knee amputee has used it with CLME. Performed tasks are walking on a treadmill and alternating stair ascent and descent. The subject was able to walk on the treadmill at varying speeds, but needed assistance with the stairs, especially to descend. The promising results with CLME are compared with the subject's performance with her own prosthesis, the C-Leg from Otto Boc

Neuroprotective Effects of Bilobalide are Accompanied by a Reduction of Ischemia-Induced Glutamate Release \u3cem\u3ein vivo\u3c/em\u3e

Neuroprotective properties of bilobalide, a specific constituent of Ginkgo extracts, were tested in a mouse model of stroke. After 24 h of middle cerebral artery occlusion (MCAO), bilobalide reduced infarct areas in the core region (striatum) by 40–50% when given at 10 mg/kg 1 h prior to MCAO. Neuroprotection was also observed at lower doses, or when the drug was given 1 h past stroke induction. Sensorimotor function in mice was improved by bilobalide as shown by corner and chimney tests. When brain metabolism in situ was monitored by microdialysis, MCAO caused a rapid disappearance of extracellular glucose in the striatum which returned to baseline levels after reperfusion. Extracellular levels of glutamate were increased by more than ten-fold in striatal tissue, and by four- to fivefold in hippocampal tissue (penumbra). Bilobalide did not affect glucose levels but strongly attenuated glutamate release in both core and penumbra regions. Bilobalide was equally active when given locally via the microdialysis probe and also reduced ischemia-induced glutamate release in vitro in brain slices. We conclude that bilobalide is a strong neuroprotectant in vivo at doses that can be used therapeutically in humans. The mechanism of action evidently involves reduction of glutamate release, thereby reducing excitotoxicity

Schulische Bildung unter Berücksichtigung von Digitalität – in Präsenz und Distanz

Wie kann schulische Bildung in einer digitalisierten Welt in der Sekundarstufe I der Laborschule verstanden werden? Wie ordnen sich Kritik- und Demokratiefähigkeit sowie Mündigkeit in einer digitalisierten Welt in dieses Verständnis ein? Wie sollen vor diesem Hintergrund digitale Medien in den Unterrichts- und Schulalltag integriert werden? Mit diesen Fragen hat das Forschungs- und Entwicklungsprojekt „Bildung in einer digitalisierten Welt im Unterrichts- und Schulalltag der Sekundarstufe I der Laborschule“ Anfang 2020 die Arbeit aufgenommen und sich auf den Weg gemacht, das Medienkonzept der Laborschule Bielefeld (weiter) zu entwickeln. Dieser Beitrag zeichnet nach, wie die COVID-19-Pandemie den Forschungszugang der Gruppe verändert und wie die Ergebnisse aus den in den Pandemiejahren durchgeführten Studien für das ursprüngliche Ziel, die Weiterentwicklung des Medienkonzeptes, nutzbar gemacht werden

Data-based school improvement. Direct and indirect effects of integrative leadership on teachers\u27 data use

Die vorliegende Studie untersucht die Zusammenhänge zwischen integrativem Schulleitungshandeln, das transformationale und instruktionale Komponenten enthält, und der Nutzungshäufigkeit verschiedener Datenquellen durch Lehrkräfte. Die Ergebnisse eines Strukturgleichungsmodells zeigen, dass integrative Führung direkte und indirekte Zusammenhänge mit der Nutzung verschiedener Datenquellen aufweist. Die Effekte scheinen vorwiegend durch die Kooperationsaktivität der Lehrkräfte vermittelt zu sein. (DIPF/Orig.)The present article reports the effects of school principals’ integrative leadership behavior, including transformational and instructional components, on teachers’ data use. Results of a structural equation model indicate significant positive direct and indirect effects on teachers’ use of various data. Total effects seem to be mainly mediated by teachers’ cooperation activities. (DIPF/Orig.

Resuspendable Powders of Lyophilized Chalcogen Particles with Activity against Microorganisms

Many organic sulfur, selenium and tellurium compounds show considerable activity against microorganisms, including bacteria and fungi. This pronounced activity is often due to the specific, oxidizing redox behavior of the chalcogen-chalcogen bond present in such molecules. Interestingly, similar chalcogen-chalcogen motifs are also found in the elemental forms of these elements, and while those materials are insoluble in aqueous media, it has recently been possible to unlock their biological activities using naturally produced or homogenized suspensions of respective chalcogen nanoparticles. Those suspensions can be employed readily and often effectively against common pathogenic microorganisms, still their practical uses are limited as such suspensions are difficult to transport, store and apply. Using mannitol as stabilizer, it is now possible to lyophilize such suspensions to produce solid forms of the nanoparticles, which upon resuspension in water essentially retain their initial size and exhibit considerable biological activity. The sequence of Nanosizing, Lyophilization and Resuspension (NaLyRe) eventually provides access to a range of lyophilized materials which may be considered as easy-to-handle, ready-to-use and at the same time as bioavailable, active forms of otherwise insoluble or sparingly substances. In the case of elemental sulfur, selenium and tellurium, this approach promises wider practical applications, for instance in the medical or agricultural arena

Dications of 3-Phenyl-indenylidene Dibenzo[ a.d ]cycloheptene: The Role of Charge in the Antiaromaticity of Cationic Systems

Dications of 9-(3-phenyl-1H-inden-1-ylidene)-5H-dibenzo[a,d]cycloheptene, 52+, were prepared by oxidation with SbF5 in SO2ClF, and their magnetic behavior was compared to dications of 9-(3-phenyl-1H-inden-1-ylidene)-9H-fluorene, 22+. The good correlation between the experimental 1H NMR shifts for the dications that were oxidized cleanly and the chemical shifts calculated by the GAIO method supported the use of the nucleus independent chemical shifts, NICS, to evaluate the antiaromaticity of the indenyl systems of 22+/52+ and their unsubstituted parent compounds, 62+ and 72+, as well as the antiaromaticity of the fluorenyl system of 22+/72+ and the aromaticity of the dibenzotropylium system of 52+/62+. Antiaromaticity was shown to be directly related to the amount of charge in the antiaromatic systems, with the antiaromatic systems more responsive to changes in the calculated NBO charge than the aromatic systems. The antiaromaticity was also shown to be directly related to the amount of delocalization in the ring system. The aromaticity of the dibenzotropylium system was much less responsive to changes in the amount of charge in the tropylium system, because the aromatic system was much more completely delocalized. Thus, antiaromatic species are more sensitive probes of delocalization than aromatic ones